EZ-editor™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With EZ-editor™, Ubigene has
successfully edited over 10000 genes on more than 500 types of cell lines.
Objective
To create a Human HSPG2 Knockout
model in cell line by EZ-editor™-mediated genome engineering.
Target gene info
Official symbol
HSPG2
Gene id
3339
Organism
Homo sapiens
Gene type
protein-coding
Official full symbol
heparan sulfate proteoglycan 2
Also known as
HSPG, PLC, PRCAN, SJA, SJS, SJS1
Genomic regions
Chromosome 1
Summary
This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants.
Strategy Summary
This gene has 9 protein coding transcripts：
Transcript
Length(nt)
Protein
Length(aa)
Protein name
Transcript type
Transcript name
ccds
NM_005529
14341
NP_005520
4391
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant 2
CCDS30625.1
XM_047419090
14836
XP_047275046
4556
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X3
NM_001291860
14344
NP_001278789
4392
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant 1
XM_047419080
14839
XP_047275036
4557
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X2
XM_011541318
14890
XP_011539620
4574
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X1
XM_017001122
14404
XP_016856611
4438
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X7
XM_017001121
14407
XP_016856610
4439
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X6
XM_047419091
14455
XP_047275047
4455
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X5
XM_017001120
14458
XP_016856609
4456
basement membrane-specific heparan sulfate proteoglycan core protein
3
transcript variant X4
Frame-shift
Fragment A
Fragment B
gRNA Detail
Strategy
Project Comprehensive Difficulty Assessment
According to the Red Cotton database: the CRISPR gene-editing strategy design is Unknown. Knockout project comprehensive difficulty is thus assessed as Unknown.
Red Cotton™ Notes
Gene
HSPG2
had been KO in hela cell line.
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level
EZ-editor™ Gene Copy Number
EZ-editor™ Gene Dependency
Result
The HSPG2 gene you inquire is evaluated as high risk
in 0%
cell line.
Cell line is not selected, unable to assess the accurate risk level, for reference only.
In all cell lines, there is
4.8% cells with expression level below cutoff,
24.4% cells with low expression level,
70.8% cells with medium expression level
of HSPG2 gene.
Cell line is not selected, unable to assess the accurate expression level, for reference only.
In all cell lines, there is
68.3% cells with low copy number,
28.9% cells with medium copy number,
2.8% cells with high copy number
of HSPG2 gene.
Cell line is not selected, unable to assess the accurate copy number, for reference only.
Ubigene is an international high-technology enterprise focused on gene-editing cells. Our exclusive EZ-editor™ technology has 10-20 times more efficient editing than traditional methods, easily achieving gene knockout, point mutation, and knock-in. Based on EZ-editor™ technology, Ubigene has accumulated over 6000 successful gene-editing cases from more than 300 cell lines including iPSC and ESC, and has established a KO Cell Line Bank with 5000+ KO cell lines and Red Cotton™ gRNA Plasmid Bank with 10000+ gRNA plasmids available in stock.
Ubigene focuses on technological innovation and product development, of which EZ-editor™ series products that cover the whole workflow of gene-editing keep improving. Ubigene will move on toward our goal of "Make genome editing easier" and we won't stop!
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level 
EZ-editor™ Gene Copy Number 
