EZ-editor™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With EZ-editor™, Ubigene has
successfully edited over 10000 genes on more than 500 types of cell lines.
Objective
To create a Human SLC17A5 Knockout
model in cell line by EZ-editor™-mediated genome engineering.
Target gene info
Official symbol
SLC17A5
Gene id
26503
Organism
Homo sapiens
Gene type
protein-coding
Official full symbol
solute carrier family 17 member 5
Also known as
AST, ISSD, NSD, SD, SIALIN, SIASD, SLD
Genomic regions
Chromosome 6
Summary
This gene encodes a membrane transporter that exports free sialic acids that have been cleaved off of cell surface lipids and proteins from lysosomes. Mutations in this gene cause sialic acid storage diseases, including infantile sialic acid storage disorder and and Salla disease, an adult form.
Strategy Summary
This gene has 11 protein coding transcripts：
Transcript
Length(nt)
Protein
Length(aa)
Protein name
Transcript type
Transcript name
ccds
NM_001382636
3008
NP_001369565
389
sialin
3
transcript variant 9
NM_001382629
3095
NP_001369558
418
sialin
3
transcript variant 2
NM_001382632
3205
NP_001369561
466
sialin
3
transcript variant 5
NM_001382635
3289
NP_001369564
494
sialin
3
transcript variant 8
NM_001382634
3133
NP_001369563
442
sialin
3
transcript variant 7
NM_001382630
3201
NP_001369559
470
sialin
3
transcript variant 3
NM_012434
3292
NP_036566
495
sialin
3
transcript variant 1
CCDS4981.1
NM_001382633
3390
NP_001369562
533
sialin
3
transcript variant 6
NM_001382631
3313
NP_001369560
502
sialin
3
transcript variant 4
XM_047418631
2807
XP_047274587
364
sialin
3
transcript variant X2
XM_047418630
1308
XP_047274586
397
sialin
3
transcript variant X1
Frame-shift
Fragment A
Fragment B
gRNA Detail
Strategy
Project Comprehensive Difficulty Assessment
According to the Red Cotton database: the CRISPR gene-editing strategy design is Unknown. Knockout project comprehensive difficulty is thus assessed as Unknown.
Red Cotton™ Notes
Gene
SLC17A5
had been KO in hela cell line.
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level
EZ-editor™ Gene Copy Number
EZ-editor™ Gene Dependency
Result
The SLC17A5 gene you inquire is evaluated as high risk
in 0%
cell line.
Cell line is not selected, unable to assess the accurate risk level, for reference only.
In all cell lines, there is
0.1% cells with expression level below cutoff,
58.9% cells with low expression level,
41.0% cells with medium expression level
of SLC17A5 gene.
Cell line is not selected, unable to assess the accurate expression level, for reference only.
In all cell lines, there is
65.1% cells with low copy number,
30.7% cells with medium copy number,
4.2% cells with high copy number
of SLC17A5 gene.
Cell line is not selected, unable to assess the accurate copy number, for reference only.
Ubigene is an international high-technology enterprise focused on gene-editing cells. Our exclusive CRISPR-U™ technology has 10-20 times more efficient editing than traditional methods, easily achieving gene knockout, point mutation, and knock-in. Based on CRISPR-U™ technology, Ubigene has accumulated over 6000 successful gene-editing cases from more than 300 cell lines including iPSC and ESC, and has established a KO Cell Line Bank with 5000+ KO cell lines and Red Cotton™ gRNA Plasmid Bank with 10000+ gRNA plasmids available in stock.
Ubigene focuses on technological innovation and product development, of which EZ-editor™ series products that cover the whole workflow of gene-editing keep improving. Ubigene will move on toward our goal of "Make genome editing easier" and we won't stop!
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level 
EZ-editor™ Gene Copy Number 
