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EZ-editor™ Gene Knockout Cell Line Strategy

Tpm1 Gene Knockout Strategy

EZ-editor™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With EZ-editor™, Ubigene has successfully edited over 10000 genes on more than 500 types of cell lines.
Objective
To create a Rat Tpm1 Knockout model in cell line by EZ-editor™-mediated genome engineering.
Target gene info
Official symbol
Tpm1
Gene id
24851
Organism
Rattus norvegicus
Gene type
protein-coding
Official full symbol
tropomyosin 1
Also known as
Alpha-tm, Tma2, Tmsa
Genomic regions
Chromosome 8
Summary
This gene is a member of the tropomyosin family and encodes a protein that heterodimerizes with a beta subunit, binding actin in both muscle and nonmuscle cells. In both smooth and striated muscle cells, it plays a role in calcium dependent-regulation of muscle contraction. In nonmuscle cells, it stabilizes cytoskeleton actin filaments. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Strategy Summary
This gene has 28 protein coding transcripts：
Transcript Length(nt) Protein Length(aa) Protein name Transcript type Transcript name ccds
XM_063264927 3072 XP_063120997 281 tropomyosin alpha-1 chain 3 transcript variant X6
XM_006243390 1899 XP_006243452 284 tropomyosin alpha-1 chain 3 transcript variant X2
XM_063264926 2995 XP_063120996 281 tropomyosin alpha-1 chain 3 transcript variant X5
XM_063264928 2995 XP_063120998 281 tropomyosin alpha-1 chain 3 transcript variant X7
XM_063264930 2025 XP_063121000 326 tropomyosin alpha-1 chain 3 transcript variant X11
NM_001034069 1740 NP_001029241 284 tropomyosin alpha-1 chain 3 transcript variant Tpm1.6
NM_001034070 1740 NP_001029242 284 tropomyosin alpha-1 chain 3 transcript variant Tpm1.7
NM_001034071 1740 NP_001029243 284 tropomyosin alpha-1 chain 3 transcript variant Tpm1.3
XM_006243395 2860 XP_006243457 245 tropomyosin alpha-1 chain 3 transcript variant X10
NM_001034073 1677 NP_001029245 248 tropomyosin alpha-1 chain 3 transcript variant Tpm1.8
NM_001034074 1677 NP_001029246 248 tropomyosin alpha-1 chain 3 transcript variant Tpm1.9
NM_001034072 1261 NP_001029244 281 tropomyosin alpha-1 chain 3 transcript variant Tpm1.10
NM_001301736 1188 NP_001288665 245 tropomyosin alpha-1 chain 3 transcript variant Tpm1.12
NM_001034068 1166 NP_001029240 287 tropomyosin alpha-1 chain 3 transcript variant 1
NM_001301336 1235 NP_001288265 284 tropomyosin alpha-1 chain 3 transcript variant Tpm1.1
NM_001301342 1251 NP_001288271 284 tropomyosin alpha-1 chain 3 transcript variant Tpm1.2
XM_063264935 1190 XP_063121005 251 tropomyosin alpha-1 chain 3 transcript variant X16
NM_019131 1103 NP_062004 251 tropomyosin alpha-1 chain 3 transcript variant Tpm1.11
NM_001034075 1182 NP_001029247 248 tropomyosin alpha-1 chain 3 transcript variant Tpm1.13
XM_063264933 1320 XP_063121003 287 tropomyosin alpha-1 chain 3 transcript variant X14
XM_008766207 1399 XP_008764429 284 tropomyosin alpha-1 chain 3 transcript variant X1
XM_063264934 1243 XP_063121004 287 tropomyosin alpha-1 chain 3 transcript variant X15
XM_063264924 1322 XP_063120994 284 tropomyosin alpha-1 chain 3 transcript variant X3
XM_063264929 1187 XP_063120999 248 tropomyosin alpha-1 chain 3 transcript variant X8
XM_063264932 2062 XP_063121002 326 tropomyosin alpha-1 chain 3 transcript variant X13
XM_006243393 1801 XP_006243455 248 tropomyosin alpha-1 chain 3 transcript variant X9
XM_063264925 1130 XP_063120995 284 tropomyosin alpha-1 chain 3 transcript variant X4
XM_063264931 1256 XP_063121001 326 tropomyosin alpha-1 chain 3 transcript variant X12
Frame-shift
Fragment A
Fragment B
Strategy

综合难度评估

根据源井生物红棉数据库显示：CRISPR基因编辑方案未知,
KO项目综合难度评估为：未知。
Red Cotton™ Notes Gene Tpm1 had been KO in hela cell line.
EZ-editor™基因风险评估
EZ-editor™基因表达量
EZ-editor™基因拷贝数

EZ-editor™基因风险评估

评估结果

Tpm1基因在0%的细胞中属高风险， 由于您未选择具体细胞，我们无法提供精准分数，仅供参考。

基因致死分数

Dependent Cell Lines Gene Dependency 0 0.5 1

不同细胞中Tpm1基因致死分数

0.0%>0.5
所有细胞
0分
非致死基因
0.5分
界限
1分
致死基因
查看详情

EZ-editor™基因表达量

表达结果

Tpm1基因在 所有细胞中有 0.1%细胞不表达， 9.9%细胞低表达， 78.8%细胞中表达， 11.1%细胞高表达， 由于您未选择具体细胞，我们无法提供精准分数，仅供参考。

基因的表达量

Cell Lines Frequency log2(TPM+1) 0 2 4 6 8 10 12

不同细胞中Tpm1基因表达量TPM

0.1%9.9%78.8%11.1%
所有细胞
0~0.5
不表达
0.5~10
低表达
10~1000
中等
>1000
高表达
查看详情

EZ-editor™基因拷贝数

评估结果

Tpm1基因在 所有细胞中有 64.9%细胞拷贝数较低， 31.5%细胞拷贝数中等， 3.6%细胞拷贝数较高， 由于您未选择具体细胞，我们无法提供精准拷贝数，仅供参考。

基因的拷贝数

Copy number 0 1 2 3 4 5 6 7 0 100 200 300 400 500 600Cell Lines

不同细胞中Tpm1基因拷贝数

64.9%31.5%3.6%
所有细胞
0~2
低拷贝数
2~4
中拷贝数
>4
高拷贝数
查看详情
Work flow
Ubigene Red Cotton Workflow

“源”于此，不止于此

源井生物是一家专注于细胞基因编辑的国际化高新技术企业，独家CRISPR-U™专利技术，编辑效率较传统方法高出10-20倍，可轻松完成细胞的基因敲除、点突变与敲入。基于CRISPR-U™技术，源井生物已积累300多种细胞（含iPS/ES）超6000个基因编辑的成功案例，并拥有5000+KO细胞资源库和上万条 gRNA载体现货库。

源井生物注重技术创新和产品开发，其覆盖基因编辑全流程的EZ-editor™系列产品仍在不断完善，向着“让基因编辑更简单”的企业目标砥砺前行，永不止步！

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温馨提示 ✖

红棉系统新技能上线啦！基因风险评估：“火眼金睛”辨基因，致死风险一秒知。评估结果可在Red Cotton Assessment模块查看哦。

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