CRISPR-U™ Gene Knockout Cell Line Strategy

SIGMAR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
To create a Human SIGMAR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SIGMAR1
Gene id 10280
Organism Homo sapiens
Official full symbol sigma non-opioid intracellular receptor 1
Gene type protein-coding
Also known as ALS16, DSMA2, OPRS1, SIG-1R, SR-BP, SR-BP1, SRBP, hSigmaR1, sigma1R
Summary This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.
Genomic regions Chromosome 9
Strategy Summary
This gene has 7 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000277010.9 SIGMAR1-201 1672 223aa Protein coding CCDS6562 Q99720-1 NM_005866.4 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000378892.5 SIGMAR1-203 1743 134aa Protein coding Q5T1J1 - GENCODE basic, TSL:2,
ENST00000679597.1 SIGMAR1-208 1612 203aa Protein coding Q99720-2 - GENCODE basic,
ENST00000680730.1 SIGMAR1-212 1457 110aa Protein coding A0A7P0T9D5 - -
ENST00000680244.1 SIGMAR1-210 864 155aa Protein coding B4DR71 - GENCODE basic,
ENST00000477726.1 SIGMAR1-205 840 192aa Protein coding CCDS6563 Q99720-3 - GENCODE basic, TSL:1,
ENST00000680277.1 SIGMAR1-211 630 159aa Protein coding A0A7P0Z4C2 - GENCODE basic,
ENST00000680104.1 SIGMAR1-209 2263 150aa Nonsense mediated decay A0A7P0TA12 - -
ENST00000353468.4 SIGMAR1-202 1582 106aa Nonsense mediated decay Q99720-4 - TSL:1,
ENST00000461426.1 SIGMAR1-204 939 No protein Protein coding CDS not defined - - TSL:1,
ENST00000478146.1 SIGMAR1-206 485 No protein Protein coding CDS not defined - - TSL:2,
ENST00000681409.1 SIGMAR1-213 2878 No protein Retained intron - - -
ENST00000497006.2 SIGMAR1-207 1842 No protein Retained intron - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SIGMAR1
This KO Strategy loading
Red Cotton™ Notes Gene SIGMAR1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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