SIGMAR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human SIGMAR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	SIGMAR1
Gene id	10280
Organism	Homo sapiens
Official full symbol	sigma non-opioid intracellular receptor 1
Gene type	protein-coding
Also known as	ALS16, DSMA2, OPRS1, SIG-1R, SR-BP, SR-BP1, SRBP, hSigmaR1, sigma1R
Summary	This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.
Genomic regions	Chromosome 9

Strategy Summary

This gene has 7 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000277010.9	SIGMAR1-201	1672	223aa	Protein coding	CCDS6562	Q99720-1	NM_005866.4	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000378892.5	SIGMAR1-203	1743	134aa	Protein coding		Q5T1J1	-	GENCODE basic, TSL:2,
ENST00000679597.1	SIGMAR1-208	1612	203aa	Protein coding		Q99720-2	-	GENCODE basic,
ENST00000680730.1	SIGMAR1-212	1457	110aa	Protein coding		A0A7P0T9D5	-	-
ENST00000680244.1	SIGMAR1-210	864	155aa	Protein coding		B4DR71	-	GENCODE basic,
ENST00000477726.1	SIGMAR1-205	840	192aa	Protein coding	CCDS6563	Q99720-3	-	GENCODE basic, TSL:1,
ENST00000680277.1	SIGMAR1-211	630	159aa	Protein coding		A0A7P0Z4C2	-	GENCODE basic,
ENST00000680104.1	SIGMAR1-209	2263	150aa	Nonsense mediated decay		A0A7P0TA12	-	-
ENST00000353468.4	SIGMAR1-202	1582	106aa	Nonsense mediated decay		Q99720-4	-	TSL:1,
ENST00000461426.1	SIGMAR1-204	939	No protein	Protein coding CDS not defined		-	-	TSL:1,
ENST00000478146.1	SIGMAR1-206	485	No protein	Protein coding CDS not defined		-	-	TSL:2,
ENST00000681409.1	SIGMAR1-213	2878	No protein	Retained intron		-	-	-
ENST00000497006.2	SIGMAR1-207	1842	No protein	Retained intron		-	-	TSL:3,

Strategy Click to get

Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	SIGMAR1
This KO Strategy	loading
Red Cotton™ Notes	Gene SIGMAR1 had been KO in a549 cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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