CDKN1A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human CDKN1A Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	CDKN1A
Gene id	1026
Organism	Homo sapiens
Official full symbol	cyclin dependent kinase inhibitor 1A
Gene type	protein-coding
Also known as	CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
Summary	This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Genomic regions	Chromosome 6

Strategy Summary

This gene has 5 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000244741.10	CDKN1A-201	2117	164aa	Protein coding	CCDS4824	A0A024RCX5 P38936	NM_000389.5	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000405375.5	CDKN1A-203	2267	164aa	Protein coding	CCDS4824	A0A024RCX5 P38936	-	GENCODE basic, APPRIS P1, TSL:1,
ENST00000448526.6	CDKN1A-204	2104	164aa	Protein coding	CCDS4824	A0A024RCX5 P38936	-	GENCODE basic, APPRIS P1, TSL:3,
ENST00000615513.4	CDKN1A-208	2102	164aa	Protein coding	CCDS4824	A0A024RCX5 P38936	-	GENCODE basic, APPRIS P1, TSL:2,
ENST00000373711.3	CDKN1A-202	793	164aa	Protein coding	CCDS4824	A0A024RCX5 P38936	-	GENCODE basic, APPRIS P1, TSL:5,
ENST00000478800.1	CDKN1A-207	616	No protein	Protein coding CDS not defined		-	-	TSL:2,
ENST00000459970.1	CDKN1A-205	604	No protein	Protein coding CDS not defined		-	-	TSL:5,
ENST00000462537.3	CDKN1A-206	553	No protein	Protein coding CDS not defined		-	-	TSL:3,

Strategy

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Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	CDKN1A
This KO Strategy	loading
Red Cotton™ Notes	Gene CDKN1A had been KO in hct116 cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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