CRISPR-U™ Gene Knockout Cell Line Strategy

CDK8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
To create a Human CDK8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK8
Gene id 1024
Organism Homo sapiens
Official full symbol cyclin dependent kinase 8
Gene type protein-coding
Also known as IDDHBA, K35
Summary This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 13
Strategy Summary
This gene has 3 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000381527.8 CDK8-201 3065 464aa Protein coding CCDS9317 P49336-1 NM_001260.3 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000625988.1 CDK8-206 795 148aa Protein coding A0A0D9SEP3 - TSL:5, CDS 5' incomplete,
ENST00000465820.6 CDK8-202 586 195aa Protein coding A0A0D9SFZ2 - TSL:3, CDS 5' and 3' incomplete,
ENST00000536792.5 CDK8-205 3032 164aa Nonsense mediated decay F5H6D4 - TSL:1,
ENST00000700501.1 CDK8-207 2989 81aa Nonsense mediated decay - - -
ENST00000700502.1 CDK8-208 2484 No protein Protein coding CDS not defined - - -
ENST00000700505.1 CDK8-211 1850 No protein Protein coding CDS not defined - - -
ENST00000700503.1 CDK8-209 1640 No protein Protein coding CDS not defined - - -
ENST00000700504.1 CDK8-210 1205 No protein Protein coding CDS not defined - - -
ENST00000480323.1 CDK8-204 1996 No protein Retained intron - - TSL:2,
ENST00000477277.5 CDK8-203 1770 No protein Retained intron - - TSL:3,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK8
This KO Strategy loading
Red Cotton™ Notes Gene CDK8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

Chick here to get more infomation>

EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

Chick here to get more infomation>

"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

Chick here to get more infomation>

Please leave your suggestion ×


红棉系统新技能上线啦!基因风险评估:“火眼金睛”辨基因,致死风险一秒知。评估结果可在Red Cotton Assessment模块查看哦。