CDK8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human CDK8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	CDK8
Gene id	1024
Organism	Homo sapiens
Official full symbol	cyclin dependent kinase 8
Gene type	protein-coding
Also known as	IDDHBA, K35
Summary	This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Genomic regions	Chromosome 13

Strategy Summary

This gene has 3 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000381527.8	CDK8-201	3065	464aa	Protein coding	CCDS9317	P49336-1	NM_001260.3	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000625988.1	CDK8-206	795	148aa	Protein coding		A0A0D9SEP3	-	TSL:5, CDS 5' incomplete,
ENST00000465820.6	CDK8-202	586	195aa	Protein coding		A0A0D9SFZ2	-	TSL:3, CDS 5' and 3' incomplete,
ENST00000536792.5	CDK8-205	3032	164aa	Nonsense mediated decay		F5H6D4	-	TSL:1,
ENST00000700501.1	CDK8-207	2989	81aa	Nonsense mediated decay		-	-	-
ENST00000700502.1	CDK8-208	2484	No protein	Protein coding CDS not defined		-	-	-
ENST00000700505.1	CDK8-211	1850	No protein	Protein coding CDS not defined		-	-	-
ENST00000700503.1	CDK8-209	1640	No protein	Protein coding CDS not defined		-	-	-
ENST00000700504.1	CDK8-210	1205	No protein	Protein coding CDS not defined		-	-	-
ENST00000480323.1	CDK8-204	1996	No protein	Retained intron		-	-	TSL:2,
ENST00000477277.5	CDK8-203	1770	No protein	Retained intron		-	-	TSL:3,

Strategy

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Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	CDK8
This KO Strategy	loading
Red Cotton™ Notes	Gene CDK8 had been KO in hela cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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