CRISPR-U™ Gene Knockout Cell Line Strategy

HNRNPR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
To create a Human HNRNPR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HNRNPR
Gene id 10236
Organism Homo sapiens
Official full symbol heterogeneous nuclear ribonucleoprotein R
Gene type protein-coding
Also known as HNRPR, hnRNP-R
Summary This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10.
Genomic regions Chromosome 1
Strategy Summary
This gene has 10 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000302271.11 HNRNPR-201 7751 633aa Protein coding CCDS232 O43390-1 NM_005826.5 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P3, TSL:1,
ENST00000478691.5 HNRNPR-210 7646 535aa Protein coding CCDS60020 O43390-4 - GENCODE basic, TSL:1,
ENST00000374612.5 HNRNPR-202 2694 633aa Protein coding CCDS232 O43390-1 - GENCODE basic, APPRIS P3, TSL:5,
ENST00000374616.7 HNRNPR-203 2691 636aa Protein coding CCDS44085 O43390-2 - GENCODE basic, APPRIS ALT1, TSL:1,
ENST00000675048.1 HNRNPR-214 2099 481aa Protein coding A0A6Q8PH35 - GENCODE basic,
ENST00000606561.5 HNRNPR-212 1837 494aa Protein coding CCDS72726 B4DT28 - GENCODE basic, TSL:2,
ENST00000427764.3 HNRNPR-204 1788 595aa Protein coding CCDS72727 O43390-3 - GENCODE basic, TSL:1,
ENST00000476451.2 HNRNPR-208 1032 238aa Protein coding A0A6Q8PEX7 - TSL:4, CDS 3' incomplete,
ENST00000463552.5 HNRNPR-205 837 279aa Protein coding A0A6Q8PH31 - TSL:4, CDS 5' and 3' incomplete,
ENST00000470941.5 HNRNPR-207 560 186aa Protein coding A0A6Q8PHG0 - TSL:4, CDS 5' and 3' incomplete,
ENST00000641107.1 HNRNPR-213 646 141aa Nonsense mediated decay A0A286YEZ8 - CDS 5' incomplete,
ENST00000476660.1 HNRNPR-209 1637 No protein Protein coding CDS not defined - - TSL:1,
ENST00000464516.1 HNRNPR-206 557 No protein Protein coding CDS not defined - - TSL:2,
ENST00000490652.1 HNRNPR-211 612 No protein Retained intron - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HNRNPR
This KO Strategy loading
Red Cotton™ Notes Gene HNRNPR had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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