HNRNPR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human HNRNPR Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	HNRNPR
Gene id	10236
Organism	Homo sapiens
Official full symbol	heterogeneous nuclear ribonucleoprotein R
Gene type	protein-coding
Also known as	HNRPR, hnRNP-R
Summary	This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10.
Genomic regions	Chromosome 1

Strategy Summary

This gene has 10 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000302271.11	HNRNPR-201	7751	633aa	Protein coding	CCDS232	O43390-1	NM_005826.5	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P3, TSL:1,
ENST00000478691.5	HNRNPR-210	7646	535aa	Protein coding	CCDS60020	O43390-4	-	GENCODE basic, TSL:1,
ENST00000374612.5	HNRNPR-202	2694	633aa	Protein coding	CCDS232	O43390-1	-	GENCODE basic, APPRIS P3, TSL:5,
ENST00000374616.7	HNRNPR-203	2691	636aa	Protein coding	CCDS44085	O43390-2	-	GENCODE basic, APPRIS ALT1, TSL:1,
ENST00000675048.1	HNRNPR-214	2099	481aa	Protein coding		A0A6Q8PH35	-	GENCODE basic,
ENST00000606561.5	HNRNPR-212	1837	494aa	Protein coding	CCDS72726	B4DT28	-	GENCODE basic, TSL:2,
ENST00000427764.3	HNRNPR-204	1788	595aa	Protein coding	CCDS72727	O43390-3	-	GENCODE basic, TSL:1,
ENST00000476451.2	HNRNPR-208	1032	238aa	Protein coding		A0A6Q8PEX7	-	TSL:4, CDS 3' incomplete,
ENST00000463552.5	HNRNPR-205	837	279aa	Protein coding		A0A6Q8PH31	-	TSL:4, CDS 5' and 3' incomplete,
ENST00000470941.5	HNRNPR-207	560	186aa	Protein coding		A0A6Q8PHG0	-	TSL:4, CDS 5' and 3' incomplete,
ENST00000641107.1	HNRNPR-213	646	141aa	Nonsense mediated decay		A0A286YEZ8	-	CDS 5' incomplete,
ENST00000476660.1	HNRNPR-209	1637	No protein	Protein coding CDS not defined		-	-	TSL:1,
ENST00000464516.1	HNRNPR-206	557	No protein	Protein coding CDS not defined		-	-	TSL:2,
ENST00000490652.1	HNRNPR-211	612	No protein	Retained intron		-	-	TSL:2,

Strategy Click to get

Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	HNRNPR
This KO Strategy	loading
Red Cotton™ Notes	Gene HNRNPR had been KO in hek293t cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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