MSLN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human MSLN Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	MSLN
Gene id	10232
Organism	Homo sapiens
Official full symbol	mesothelin
Gene type	protein-coding
Also known as	MPF, SMRP
Summary	This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Genomic regions	Chromosome 16

Strategy Summary

This gene has 8 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000545450.7	MSLN-202	2197	622aa	Protein coding	CCDS45370	Q13421-3	NM_005823.6	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P2, TSL:1,
ENST00000566549.5	MSLN-207	2418	622aa	Protein coding	CCDS45370	Q13421-3	-	GENCODE basic, APPRIS P2, TSL:1,
ENST00000382862.7	MSLN-201	2116	630aa	Protein coding	CCDS32356	Q13421-1	-	GENCODE basic, APPRIS ALT2, TSL:5,
ENST00000563941.5	MSLN-205	2109	622aa	Protein coding	CCDS45370	Q13421-3	-	GENCODE basic, APPRIS P2, TSL:1,
ENST00000561896.1	MSLN-203	1195	398aa	Protein coding		H3BUX1	-	TSL:5, CDS 5' and 3' incomplete,
ENST00000563651.5	MSLN-204	1084	332aa	Protein coding		H3BR90	-	TSL:5, CDS 3' incomplete,
ENST00000566269.6	MSLN-206	1068	334aa	Protein coding		H3BMA1	-	TSL:3, CDS 5' incomplete,
ENST00000569566.2	MSLN-208	550	170aa	Protein coding		H3BV92	-	TSL:3, CDS 3' incomplete,

Strategy Click to get

Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	MSLN
This KO Strategy	loading
Red Cotton™ Notes	Gene MSLN had been KO in hela cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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