CRISPR-U™ Gene Knockout Cell Line Strategy

MSLN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
To create a Human MSLN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MSLN
Gene id 10232
Organism Homo sapiens
Official full symbol mesothelin
Gene type protein-coding
Also known as MPF, SMRP
Summary This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Genomic regions Chromosome 16
Strategy Summary
This gene has 8 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000545450.7 MSLN-202 2197 622aa Protein coding CCDS45370 Q13421-3 NM_005823.6 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P2, TSL:1,
ENST00000566549.5 MSLN-207 2418 622aa Protein coding CCDS45370 Q13421-3 - GENCODE basic, APPRIS P2, TSL:1,
ENST00000382862.7 MSLN-201 2116 630aa Protein coding CCDS32356 Q13421-1 - GENCODE basic, APPRIS ALT2, TSL:5,
ENST00000563941.5 MSLN-205 2109 622aa Protein coding CCDS45370 Q13421-3 - GENCODE basic, APPRIS P2, TSL:1,
ENST00000561896.1 MSLN-203 1195 398aa Protein coding H3BUX1 - TSL:5, CDS 5' and 3' incomplete,
ENST00000563651.5 MSLN-204 1084 332aa Protein coding H3BR90 - TSL:5, CDS 3' incomplete,
ENST00000566269.6 MSLN-206 1068 334aa Protein coding H3BMA1 - TSL:3, CDS 5' incomplete,
ENST00000569566.2 MSLN-208 550 170aa Protein coding H3BV92 - TSL:3, CDS 3' incomplete,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MSLN
This KO Strategy loading
Red Cotton™ Notes Gene MSLN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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