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CRISPR-U™ Gene Knockout Cell Line Strategy

CTDSPL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human CTDSPL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CTDSPL
Gene id 10217
Organism Homo sapiens
Official full symbol CTD small phosphatase like
Gene type protein-coding
Also known as C3orf8, HYA22, PSR1, RBSP3, SCP3
Genomic regions Chromosome 3
Strategy Summary
This gene has 5 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000273179.10 CTDSPL-201 4753 276aa Protein coding CCDS33734 O15194-1 NM_001008392.2 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P4, TSL:1,
ENST00000443503.6 CTDSPL-206 4640 265aa Protein coding CCDS33735 O15194-2 - GENCODE basic, APPRIS ALT1, TSL:1,
ENST00000436654.1 CTDSPL-205 804 103aa Protein coding H7C353 - TSL:3, CDS 5' incomplete,
ENST00000447745.5 CTDSPL-207 793 196aa Protein coding H7C2S4 - TSL:3, CDS 5' incomplete,
ENST00000416688.5 CTDSPL-203 368 93aa Protein coding H7C2B9 - TSL:3, CDS 5' incomplete,
ENST00000435525.1 CTDSPL-204 371 55aa Nonsense mediated decay F8WED2 - TSL:3,
ENST00000310189.3 CTDSPL-202 1482 No protein Protein coding CDS not defined - - TSL:5,
ENST00000486978.5 CTDSPL-208 609 No protein Protein coding CDS not defined - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CTDSPL
This KO Strategy loading
Red Cotton™ Notes Gene CTDSPL had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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温馨提示

红棉系统新技能上线啦!基因风险评估:“火眼金睛”辨基因,致死风险一秒知。评估结果可在Red Cotton Assessment模块查看哦。

评估结果是基于Achilles和Sanger数据库公布的CRISPR全基因组文库筛选细胞数据,与单个基因敲除实验结果可能不同,仅供参考。