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CRISPR-U™ Gene Knockout Cell Line Strategy

TANK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human TANK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TANK
Gene id 10010
Organism Homo sapiens
Official full symbol TRAF family member associated NFKB activator
Gene type protein-coding
Also known as I-TRAF, ITRAF, TRAF2
Summary The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 12 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000392749.7 TANK-202 2098 425aa Protein coding CCDS2215 Q92844 NM_001199135.3 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000405852.5 TANK-205 2155 408aa Protein coding E7EQA9 - GENCODE basic, TSL:5,
ENST00000259075.6 TANK-201 2152 425aa Protein coding CCDS2215 Q92844 - GENCODE basic, APPRIS P1, TSL:1,
ENST00000437623.1 TANK-210 1702 275aa Protein coding H7C3L4 - TSL:5, CDS 5' incomplete,
ENST00000402568.5 TANK-203 878 239aa Protein coding E7EW82 - GENCODE basic, TSL:5,
ENST00000406287.5 TANK-206 875 238aa Protein coding E7EVA2 - GENCODE basic, TSL:5,
ENST00000429217.5 TANK-207 762 190aa Protein coding E7ENY2 - TSL:5, CDS 3' incomplete,
ENST00000439442.1 TANK-211 727 198aa Protein coding H7BZ28 - TSL:3, CDS 5' incomplete,
ENST00000432002.5 TANK-208 600 138aa Protein coding E7EV32 - TSL:5, CDS 3' incomplete,
ENST00000456358.5 TANK-214 564 165aa Protein coding E7EMT6 - TSL:5, CDS 3' incomplete,
ENST00000403609.1 TANK-204 555 119aa Protein coding CCDS46436 Q92844-3 - GENCODE basic, TSL:1,
ENST00000440506.5 TANK-212 550 105aa Protein coding E7EWR8 - TSL:3, CDS 3' incomplete,
ENST00000457887.5 TANK-215 762 128aa Nonsense mediated decay E7ERM9 - TSL:5,
ENST00000432692.6 TANK-209 576 125aa Nonsense mediated decay E7EM55 - TSL:5,
ENST00000441987.5 TANK-213 533 36aa Nonsense mediated decay E9PEK1 - TSL:5,
ENST00000461338.5 TANK-216 674 No protein Protein coding CDS not defined - - TSL:3,
ENST00000494976.1 TANK-223 636 No protein Protein coding CDS not defined - - TSL:3,
ENST00000470324.1 TANK-219 571 No protein Protein coding CDS not defined - - TSL:4,
ENST00000463502.1 TANK-217 570 No protein Protein coding CDS not defined - - TSL:3,
ENST00000489393.1 TANK-222 301 No protein Protein coding CDS not defined - - TSL:3,
ENST00000468831.5 TANK-218 905 No protein Retained intron - - TSL:1,
ENST00000483622.1 TANK-221 566 No protein Retained intron - - TSL:5,
ENST00000477952.1 TANK-220 427 No protein Retained intron - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TANK
This KO Strategy loading
Red Cotton™ Notes Gene TANK had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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