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CRISPR-U™ Gene Knockout Cell Line Strategy

GNPDA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human GNPDA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNPDA1
Gene id 10007
Organism Homo sapiens
Official full symbol glucosamine-6-phosphate deaminase 1
Gene type protein-coding
Also known as GNP1, GNPDA, GNPI, GPI, HLN
Summary Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 ).
Genomic regions Chromosome 5
Strategy Summary
This gene has 10 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000311337.11 GNPDA1-201 2267 289aa Protein coding CCDS4272 P46926 NM_005471.5 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000508177.5 GNPDA1-210 2980 289aa Protein coding CCDS4272 P46926 - GENCODE basic, APPRIS P1, TSL:1,
ENST00000503794.5 GNPDA1-204 2622 289aa Protein coding CCDS4272 P46926 - GENCODE basic, APPRIS P1, TSL:1,
ENST00000500692.6 GNPDA1-202 2319 289aa Protein coding CCDS4272 P46926 - GENCODE basic, APPRIS P1, TSL:5,
ENST00000513454.5 GNPDA1-212 980 274aa Protein coding D6R9P4 - GENCODE basic, TSL:5,
ENST00000505689.5 GNPDA1-207 925 290aa Protein coding D6RFF8 - TSL:5, CDS 3' incomplete,
ENST00000504139.5 GNPDA1-205 754 237aa Protein coding D6RAY7 - TSL:5, CDS 3' incomplete,
ENST00000510194.5 GNPDA1-211 570 160aa Protein coding D6RB13 - TSL:4, CDS 3' incomplete,
ENST00000503229.5 GNPDA1-203 543 138aa Protein coding D6R917 - TSL:5, CDS 3' incomplete,
ENST00000504424.1 GNPDA1-206 473 29aa Protein coding D6RFK5 - TSL:4, CDS 3' incomplete,
ENST00000507107.1 GNPDA1-208 556 No protein Retained intron - - TSL:2,
ENST00000515747.1 GNPDA1-213 555 No protein Retained intron - - TSL:2,
ENST00000507559.1 GNPDA1-209 413 No protein Retained intron - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNPDA1
This KO Strategy loading
Red Cotton™ Notes Gene GNPDA1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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