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CRISPR-U™ Gene Knockout Cell Line Strategy

ADA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human ADA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ADA
Gene id 100
Organism Homo sapiens
Official full symbol adenosine deaminase
Gene type protein-coding
Also known as ADA1
Summary This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
Genomic regions Chromosome 20
Strategy Summary
This gene has 25 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000372874.9 ADA-201 1496 363aa Protein coding CCDS13335 A0A0S2Z381 P00813 NM_000022.4 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P2, TSL:1,
ENST00000537820.2 ADA-207 2387 339aa Protein coding CCDS82618 F5GWI4 - GENCODE basic, TSL:1,
ENST00000696076.1 ADA-251 2032 441aa Protein coding - - GENCODE basic,
ENST00000696017.1 ADA-232 1927 417aa Protein coding - - GENCODE basic,
ENST00000536076.2 ADA-205 1690 312aa Protein coding - - GENCODE basic, TSL:4,
ENST00000696064.1 ADA-245 1645 312aa Protein coding - - GENCODE basic,
ENST00000695927.1 ADA-213 1630 389aa Protein coding - - GENCODE basic,
ENST00000695993.1 ADA-219 1612 387aa Protein coding - - GENCODE basic,
ENST00000696060.1 ADA-241 1600 386aa Protein coding - - GENCODE basic,
ENST00000696058.1 ADA-239 1578 386aa Protein coding - - GENCODE basic,
ENST00000696078.1 ADA-253 1537 362aa Protein coding - - GENCODE basic, APPRIS ALT1,
ENST00000696061.1 ADA-242 1533 362aa Protein coding - - GENCODE basic, APPRIS ALT1,
ENST00000696077.1 ADA-252 1529 361aa Protein coding - - GENCODE basic, APPRIS ALT1,
ENST00000696063.1 ADA-244 1517 388aa Protein coding - - GENCODE basic,
ENST00000696080.1 ADA-255 1502 400aa Protein coding - - GENCODE basic,
ENST00000696062.1 ADA-243 1487 384aa Protein coding - - GENCODE basic,
ENST00000696082.1 ADA-257 1479 388aa Protein coding - - GENCODE basic,
ENST00000696079.1 ADA-254 1464 399aa Protein coding - - GENCODE basic,
ENST00000695949.1 ADA-214 1450 338aa Protein coding - - GENCODE basic,
ENST00000696005.1 ADA-226 1305 235aa Protein coding - - CDS 5' incomplete,
ENST00000695995.1 ADA-221 1085 233aa Protein coding - - GENCODE basic,
ENST00000695991.1 ADA-217 1014 209aa Protein coding A0A0S2Z3B9 - GENCODE basic,
ENST00000695889.1 ADA-210 975 188aa Protein coding A0A0S2Z3T6 - GENCODE basic,
ENST00000696065.1 ADA-246 726 137aa Protein coding - - GENCODE basic,
ENST00000695956.1 ADA-215 572 125aa Protein coding - - CDS 5' incomplete,
ENST00000696038.1 ADA-237 1925 83aa Nonsense mediated decay - - -
ENST00000696034.1 ADA-233 1877 263aa Nonsense mediated decay - - -
ENST00000695992.1 ADA-218 1838 263aa Nonsense mediated decay - - -
ENST00000696059.1 ADA-240 1622 53aa Nonsense mediated decay - - -
ENST00000696075.1 ADA-250 1621 48aa Nonsense mediated decay - - -
ENST00000536532.6 ADA-206 1423 263aa Nonsense mediated decay F5GXW0 - TSL:5,
ENST00000695994.1 ADA-220 1383 254aa Nonsense mediated decay - - -
ENST00000696006.1 ADA-227 1362 239aa Nonsense mediated decay - - -
ENST00000696007.1 ADA-228 1301 119aa Nonsense mediated decay - - CDS 5' incomplete,
ENST00000696104.1 ADA-260 1180 204aa Nonsense mediated decay - - -
ENST00000539235.6 ADA-208 1096 75aa Nonsense mediated decay F5GYD4 - TSL:5,
ENST00000695957.1 ADA-216 1084 130aa Nonsense mediated decay - - -
ENST00000696105.1 ADA-261 848 178aa Nonsense mediated decay - - -
ENST00000695891.1 ADA-212 629 156aa Nonsense mediated decay - - -
ENST00000695890.1 ADA-211 5965 No protein Retained intron - - -
ENST00000696008.1 ADA-229 4168 No protein Retained intron - - -
ENST00000696003.1 ADA-224 4092 No protein Retained intron - - -
ENST00000696004.1 ADA-225 3536 No protein Retained intron - - -
ENST00000696037.1 ADA-236 3081 No protein Retained intron - - -
ENST00000696036.1 ADA-235 2556 No protein Retained intron - - -
ENST00000696083.1 ADA-258 2328 No protein Retained intron - - -
ENST00000696009.1 ADA-230 2186 No protein Retained intron - - -
ENST00000696035.1 ADA-234 2041 No protein Retained intron - - -
ENST00000696084.1 ADA-259 1994 No protein Retained intron - - -
ENST00000696081.1 ADA-256 1956 No protein Retained intron - - -
ENST00000696010.1 ADA-231 1831 No protein Retained intron - - -
ENST00000464097.5 ADA-202 1770 No protein Retained intron - - TSL:2,
ENST00000696073.1 ADA-248 1715 No protein Retained intron - - -
ENST00000696039.1 ADA-238 1684 No protein Retained intron - - -
ENST00000492931.6 ADA-203 1569 No protein Retained intron - - TSL:5,
ENST00000695996.1 ADA-222 1486 No protein Retained intron - - -
ENST00000696072.1 ADA-247 1210 No protein Retained intron - - -
ENST00000695997.1 ADA-223 1011 No protein Retained intron - - -
ENST00000696074.1 ADA-249 955 No protein Retained intron - - -
ENST00000535573.1 ADA-204 550 No protein Retained intron - - TSL:5,
ENST00000545776.5 ADA-209 549 No protein Retained intron - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ADA
This KO Strategy loading
Red Cotton™ Notes Gene ADA had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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