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EZ-editor™ Gene Knockout Cell Line Strategy

STAT2 Gene Knockout Strategy

EZ-editor™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With EZ-editor™, Ubigene has successfully edited over 10000 genes on more than 500 types of cell lines.
Objective
To create a Human STAT2 Knockout model in cell line by EZ-editor™-mediated genome engineering.
Target gene info
Official symbol
STAT2
Gene id
6773
Organism
Homo sapiens
Gene type
protein-coding
Official full symbol
signal transducer and activator of transcription 2
Also known as
IMD44, ISGF-3, P113, PTORCH3, STAT113
Genomic regions
Chromosome 12
Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Mutations in this gene result in Immunodeficiency 44.
Strategy Summary
This gene has 15 protein coding transcripts：
Transcript Length(nt) Protein Length(aa) Protein name Transcript type Transcript name ccds
NM_001385110 4372 NP_001372039 840 signal transducer and activator of transcription 2 3 transcript variant 3
NM_001385115 4363 NP_001372044 837 signal transducer and activator of transcription 2 3 transcript variant 7
NM_198332 4393 NP_938146 847 signal transducer and activator of transcription 2 3 transcript variant 2 CCDS55836.1
XM_011538698 4417 XP_011537000 855 signal transducer and activator of transcription 2 3 transcript variant X2
NM_001385114 4384 NP_001372043 844 signal transducer and activator of transcription 2 3 transcript variant 6
NM_001385111 4306 NP_001372040 818 signal transducer and activator of transcription 2 3 transcript variant 4 CCDS91707.1
NM_001385113 4398 NP_001372042 807 signal transducer and activator of transcription 2 3 transcript variant 5
NM_005419 4405 NP_005410 851 signal transducer and activator of transcription 2 3 transcript variant 1 CCDS8917.1
XM_011538697 4429 XP_011536999 859 signal transducer and activator of transcription 2 3 transcript variant X1
XM_047429469 3500 XP_047285425 536 signal transducer and activator of transcription 2 3 transcript variant X5
XM_011538699 2023 XP_011537001 592 signal transducer and activator of transcription 2 3 transcript variant X3
XM_047429468 1996 XP_047285424 584 signal transducer and activator of transcription 2 3 transcript variant X4
XM_047429470 1593 XP_047285426 466 signal transducer and activator of transcription 2 3 transcript variant X6
XM_047429471 1558 XP_047285427 464 signal transducer and activator of transcription 2 3 transcript variant X8
XM_047429472 1572 XP_047285428 464 signal transducer and activator of transcription 2 3 transcript variant X9
Frame-shift
Fragment A
Fragment B
Strategy

Project Comprehensive Difficulty Assessment

According to the Red Cotton database: the CRISPR gene-editing strategy design is Unknown.
Knockout project comprehensive difficulty is thus assessed as Unknown.
Red Cotton™ Notes Gene STAT2 had been KO in a549 cell line.
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level
EZ-editor™ Gene Copy Number

EZ-editor™ Gene Dependency

Result

The STAT2 gene you inquire is evaluated as high risk in 1% cell line. Cell line is not selected, unable to assess the accurate risk level, for reference only.

Gene lethality score

Dependent Cell Lines Gene Dependency 0 0.5 1

Lethality score of STAT2 in different cells

1.0%>0.5
All cells
0
Non-essential gene
0.5
Boundary score
1
Essential gene
More details

EZ-editor™ Gene Expression Level

Result

In all cell lines, there is 8.7% cells with low expression level, 91.3% cells with medium expression level of STAT2 gene. Cell line is not selected, unable to assess the accurate expression level, for reference only.

Gene Expression

Cell Lines Frequency log2(TPM+1) 0 2 4 6 8 10 12

TPM of STAT2 in different cells

8.7%91.3%
All cells
0~0.5
Below cutoff
0.5~10
Low
10~1000
Medium
>1000
High
More details

EZ-editor™ Gene Copy Number

Result

In all cell lines, there is 56.8% cells with low copy number, 38.9% cells with medium copy number, 4.3% cells with high copy number of STAT2 gene. Cell line is not selected, unable to assess the accurate copy number, for reference only.

Gene Copy Number

Copy number 0 1 2 3 4 5 6 7 0 100 200 300 400 500 600Cell Lines

Copy number of STAT2 in different cells

56.8%38.9%4.3%
All cells
0~2
Low
2~4
Medium
>4
High
More details
Work flow
Ubigene Red Cotton Workflow

Ubigene - Make genome editing easier

Ubigene is an international high-technology enterprise focused on gene-editing cells. Our exclusive EZ-editor™ technology has 10-20 times more efficient editing than traditional methods, easily achieving gene knockout, point mutation, and knock-in. Based on EZ-editor™ technology, Ubigene has accumulated over 6000 successful gene-editing cases from more than 300 cell lines including iPSC and ESC, and has established a KO Cell Line Bank with 5000+ KO cell lines and Red Cotton™ gRNA Plasmid Bank with 10000+ gRNA plasmids available in stock.

Ubigene focuses on technological innovation and product development, of which EZ-editor™ series products that cover the whole workflow of gene-editing keep improving. Ubigene will move on toward our goal of "Make genome editing easier" and we won't stop!

Cell products

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