EZ-editor™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With EZ-editor™, Ubigene has
successfully edited over 10000 genes on more than 500 types of cell lines.
Objective
To create a Human CRBN Knockout
model in cell line by EZ-editor™-mediated genome engineering.
Target gene info
Official symbol
CRBN
Gene id
51185
Organism
Homo sapiens
Gene type
protein-coding
Official full symbol
cereblon
Also known as
MRT2, MRT2A
Genomic regions
Chromosome 3
Summary
This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.
Strategy Summary
This gene has 6 protein coding transcripts：
Transcript
Length(nt)
Protein
Length(aa)
Protein name
Transcript type
Transcript name
ccds
NM_001173482
2593
NP_001166953
441
protein cereblon
3
transcript variant 2
CCDS54547.1
XM_005265202
2080
XP_005265259
379
protein cereblon
3
transcript variant X2
XM_011533791
2055
XP_011532093
398
protein cereblon
3
transcript variant X1
NM_016302
2187
NP_057386
442
protein cereblon
3
transcript variant 1
CCDS2562.1
XM_047448254
1789
XP_047304210
278
protein cereblon
3
transcript variant X4
XM_047448253
1096
XP_047304209
284
protein cereblon
3
transcript variant X3
Frame-shift
Fragment A
Fragment B
gRNA Detail
Strategy
Project Comprehensive Difficulty Assessment
According to the Red Cotton database: the CRISPR gene-editing strategy design is Unknown. Knockout project comprehensive difficulty is thus assessed as Unknown.
Red Cotton™ Notes
Gene
CRBN
had been KO in a549 cell line.
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level
EZ-editor™ Gene Copy Number
EZ-editor™ Gene Dependency
Result
The CRBN gene you inquire is evaluated as high risk
in 0%
cell line.
Cell line is not selected, unable to assess the accurate risk level, for reference only.
In all cell lines, there is
6.0% cells with low expression level,
94.0% cells with medium expression level
of CRBN gene.
Cell line is not selected, unable to assess the accurate expression level, for reference only.
In all cell lines, there is
71.6% cells with low copy number,
24.7% cells with medium copy number,
3.7% cells with high copy number
of CRBN gene.
Cell line is not selected, unable to assess the accurate copy number, for reference only.
Ubigene is an international high-technology enterprise focused on gene-editing cells. Our exclusive CRISPR-U™ technology has 10-20 times more efficient editing than traditional methods, easily achieving gene knockout, point mutation, and knock-in. Based on CRISPR-U™ technology, Ubigene has accumulated over 6000 successful gene-editing cases from more than 300 cell lines including iPSC and ESC, and has established a KO Cell Line Bank with 5000+ KO cell lines and Red Cotton™ gRNA Plasmid Bank with 10000+ gRNA plasmids available in stock.
Ubigene focuses on technological innovation and product development, of which EZ-editor™ series products that cover the whole workflow of gene-editing keep improving. Ubigene will move on toward our goal of "Make genome editing easier" and we won't stop!
EZ-editor™ Gene Dependency
EZ-editor™ Gene Expression Level 
EZ-editor™ Gene Copy Number 
