i18n.right_contact_us.contact_us
CRISPR-U™ Gene Knockout Cell Line Strategy

Serpina1e Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Mouse Serpina1e Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol
Serpina1e
Gene id
20704
Organism
Mus musculus
Gene type
protein-coding
Official full symbol
serine (or cysteine) peptidase inhibitor, clade A, member 1E
Also known as
Dom5, PI5, Spi1-5
Genomic regions
Chromosome 12
Strategy Summary
This gene has 2 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match Flags
ENSMUST00000122229.8 Serpina1e-202 1575 413aa Protein coding CCDS26142 Q00898 Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENSMUST00000085054.5 Serpina1e-201 1295 413aa Protein coding CCDS26142 Q00898 GENCODE basic, APPRIS P1, TSL:1,
Ubigene Red Cotton Transcript
Frame-shift
Fragment A
Fragment B
Strategy

Project Comprehensive Difficulty Assessment

According to the Red Cotton database: the CRISPR gene-editing strategy design is Unknown.
Knockout project comprehensive difficulty is thus assessed as Unknown.
Work flow
Ubigene Red Cotton Workflow

Ubigene - Make genome editing easier

Ubigene is an international high-technology enterprise focused on gene-editing cells. Our exclusive CRISPR-U™ technology has 10-20 times more efficient editing than traditional methods, easily achieving gene knockout, point mutation, and knock-in. Based on CRISPR-U™ technology, Ubigene has accumulated over 5000 successful gene-editing cases from more than 200 cell lines including iPSC and ESC, and has established a KO Cell Line Bank with 3500+ KO cell lines and Red Cotton™ gRNA Plasmid Bank with 10000+ gRNA plasmids available in stock.

Ubigene focuses on technological innovation and product development, of which EZ-editor™ series products that cover the whole workflow of gene-editing keep improving. Ubigene will move on toward our goal of "Make genome editing easier" and we won't stop!

Cell products

温馨提示

红棉系统新技能上线啦!基因风险评估:“火眼金睛”辨基因,致死风险一秒知。评估结果可在Red Cotton Assessment模块查看哦。