CRISPR-U™ Gene Knockout Cell Line Strategy

CDKN1B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
To create a Human CDKN1B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDKN1B
Gene id 1027
Organism Homo sapiens
Official full symbol cyclin dependent kinase inhibitor 1B
Gene type protein-coding
Also known as CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Summary This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Genomic regions Chromosome 12
Strategy Summary
This gene has 4 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000228872.9 CDKN1B-201 2411 198aa Protein coding CCDS8653 P46527 Q6I9V6 NM_004064.5 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000614874.2 CDKN1B-205 4335 198aa Protein coding CCDS8653 P46527 Q6I9V6 - GENCODE basic, APPRIS P1, TSL:NA,
ENST00000396340.1 CDKN1B-202 897 205aa Protein coding E7ES52 - GENCODE basic, TSL:3,
ENST00000442489.1 CDKN1B-203 577 104aa Protein coding H7C2T1 - TSL:5, CDS 5' incomplete,
ENST00000682620.1 CDKN1B-207 3094 No protein Protein coding CDS not defined - - -
ENST00000682080.1 CDKN1B-206 2465 No protein Protein coding CDS not defined - - -
ENST00000684771.1 CDKN1B-209 2004 No protein Protein coding CDS not defined - - -
ENST00000477087.1 CDKN1B-204 453 No protein Protein coding CDS not defined - - TSL:3,
ENST00000683707.1 CDKN1B-208 2467 No protein Retained intron - - -
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDKN1B
This KO Strategy loading
Red Cotton™ Notes Gene CDKN1B had been KO in raw cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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红棉系统新技能上线啦!基因风险评估:“火眼金睛”辨基因,致死风险一秒知。评估结果可在Red Cotton Assessment模块查看哦。