CDKN1B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human CDKN1B Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	CDKN1B
Gene id	1027
Organism	Homo sapiens
Official full symbol	cyclin dependent kinase inhibitor 1B
Gene type	protein-coding
Also known as	CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Summary	This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Genomic regions	Chromosome 12

Strategy Summary

This gene has 4 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000228872.9	CDKN1B-201	2411	198aa	Protein coding	CCDS8653	P46527 Q6I9V6	NM_004064.5	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000614874.2	CDKN1B-205	4335	198aa	Protein coding	CCDS8653	P46527 Q6I9V6	-	GENCODE basic, APPRIS P1, TSL:NA,
ENST00000396340.1	CDKN1B-202	897	205aa	Protein coding		E7ES52	-	GENCODE basic, TSL:3,
ENST00000442489.1	CDKN1B-203	577	104aa	Protein coding		H7C2T1	-	TSL:5, CDS 5' incomplete,
ENST00000682620.1	CDKN1B-207	3094	No protein	Protein coding CDS not defined		-	-	-
ENST00000682080.1	CDKN1B-206	2465	No protein	Protein coding CDS not defined		-	-	-
ENST00000684771.1	CDKN1B-209	2004	No protein	Protein coding CDS not defined		-	-	-
ENST00000477087.1	CDKN1B-204	453	No protein	Protein coding CDS not defined		-	-	TSL:3,
ENST00000683707.1	CDKN1B-208	2467	No protein	Retained intron		-	-	-

Strategy

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Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	CDKN1B
This KO Strategy	loading
Red Cotton™ Notes	Gene CDKN1B had been KO in raw cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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