ZMPSTE24 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human ZMPSTE24 Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	ZMPSTE24
Gene id	10269
Organism	Homo sapiens
Official full symbol	zinc metallopeptidase STE24
Gene type	protein-coding
Also known as	FACE-1, FACE1, HGPS, PRO1, STE24, Ste24p
Summary	This gene encodes a member of the peptidase M48A family. The encoded protein is a zinc metalloproteinase involved in the two step post-translational proteolytic cleavage of carboxy terminal residues of farnesylated prelamin A to form mature lamin A. Mutations in this gene have been associated with mandibuloacral dysplasia and restrictive dermopathy.
Genomic regions	Chromosome 1

Strategy Summary

This gene has 1 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000372759.4	ZMPSTE24-201	2975	475aa	Protein coding	CCDS449	O75844	NM_005857.5	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000675937.1	ZMPSTE24-207	3357	315aa	Nonsense mediated decay		A0A6Q8PHG9	-	-
ENST00000674703.1	ZMPSTE24-205	3263	102aa	Nonsense mediated decay		A0A6Q8PF67	-	-
ENST00000675754.1	ZMPSTE24-206	3082	124aa	Nonsense mediated decay		A0A6Q8PH40	-	-
ENST00000479131.5	ZMPSTE24-204	561	No protein	Protein coding CDS not defined		-	-	TSL:4,
ENST00000472583.1	ZMPSTE24-202	537	No protein	Protein coding CDS not defined		-	-	TSL:4,
ENST00000474142.1	ZMPSTE24-203	530	No protein	Retained intron		-	-	TSL:2,

Strategy Click to get

Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	ZMPSTE24
This KO Strategy	loading
Red Cotton™ Notes	Gene ZMPSTE24 had been KO in hela cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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