FLOT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.

Objective

To create a Human FLOT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.

Target gene info

Official symbol	FLOT1
Gene id	10211
Organism	Homo sapiens
Official full symbol	flotillin 1
Gene type	protein-coding
Summary	This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.
Genomic regions	Chromosome 6

Strategy Summary

This gene has 7 protein coding transcripts：

Transcript ID	Name	bp	Protein	Biotype	CCDS	UniProt Match	RefSeq Match	Flags
ENST00000376389.8	FLOT1-201	1866	427aa	Protein coding	CCDS4688	O75955-1	NM_005803.4	MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000438162.5	FLOT1-205	888	252aa	Protein coding		A2AB10	-	TSL:5, CDS 3' incomplete,
ENST00000413165.5	FLOT1-202	856	238aa	Protein coding		A2AB09	-	TSL:5, CDS 3' incomplete,
ENST00000445853.5	FLOT1-206	792	235aa	Protein coding		A2AB12	-	TSL:1, CDS 3' incomplete,
ENST00000454845.1	FLOT1-207	629	185aa	Protein coding		A2AB13	-	TSL:3, CDS 3' incomplete,
ENST00000416018.5	FLOT1-203	592	192aa	Protein coding		A2AB11	-	TSL:3, CDS 3' incomplete,
ENST00000418160.5	FLOT1-204	457	145aa	Protein coding		A2ABJ5	-	TSL:3, CDS 3' incomplete,
ENST00000470643.5	FLOT1-208	852	No protein	Protein coding CDS not defined		-	-	TSL:3,
ENST00000487376.5	FLOT1-212	1583	No protein	Retained intron		-	-	TSL:5,
ENST00000484168.1	FLOT1-210	1101	No protein	Retained intron		-	-	TSL:5,
ENST00000484693.1	FLOT1-211	1001	No protein	Retained intron		-	-	TSL:2,
ENST00000476729.5	FLOT1-209	562	No protein	Retained intron		-	-	TSL:2,

Strategy

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Red Cotton™ Assessment

Project Difficulty Level unknown

Target Gene	FLOT1
This KO Strategy	loading
Red Cotton™ Notes	Gene FLOT1 had been KO in hek293t cell line.

Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.

Special deals for this gene:

^$80

Single gRNA plasmid off-shelf

^$599

Single gRNA lentivirus

Work flow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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