CRISPR-U™ Gene Knockout Cell Line Strategy

FLOT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
To create a Human FLOT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FLOT1
Gene id 10211
Organism Homo sapiens
Official full symbol flotillin 1
Gene type protein-coding
Summary This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 6
Strategy Summary
This gene has 7 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000376389.8 FLOT1-201 1866 427aa Protein coding CCDS4688 O75955-1 NM_005803.4 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000438162.5 FLOT1-205 888 252aa Protein coding A2AB10 - TSL:5, CDS 3' incomplete,
ENST00000413165.5 FLOT1-202 856 238aa Protein coding A2AB09 - TSL:5, CDS 3' incomplete,
ENST00000445853.5 FLOT1-206 792 235aa Protein coding A2AB12 - TSL:1, CDS 3' incomplete,
ENST00000454845.1 FLOT1-207 629 185aa Protein coding A2AB13 - TSL:3, CDS 3' incomplete,
ENST00000416018.5 FLOT1-203 592 192aa Protein coding A2AB11 - TSL:3, CDS 3' incomplete,
ENST00000418160.5 FLOT1-204 457 145aa Protein coding A2ABJ5 - TSL:3, CDS 3' incomplete,
ENST00000470643.5 FLOT1-208 852 No protein Protein coding CDS not defined - - TSL:3,
ENST00000487376.5 FLOT1-212 1583 No protein Retained intron - - TSL:5,
ENST00000484168.1 FLOT1-210 1101 No protein Retained intron - - TSL:5,
ENST00000484693.1 FLOT1-211 1001 No protein Retained intron - - TSL:2,
ENST00000476729.5 FLOT1-209 562 No protein Retained intron - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FLOT1
This KO Strategy loading
Red Cotton™ Notes Gene FLOT1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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"8+3" CRISPR Library

Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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红棉系统新技能上线啦!基因风险评估:“火眼金睛”辨基因,致死风险一秒知。评估结果可在Red Cotton Assessment模块查看哦。