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CRISPR-U™ Gene Knockout Cell Line Strategy

PDCD6IP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human PDCD6IP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDCD6IP
Gene id 10015
Organism Homo sapiens
Official full symbol programmed cell death 6 interacting protein
Gene type protein-coding
Also known as AIP1, ALIX, DRIP4, HP95
Summary This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
Genomic regions Chromosome 3
Strategy Summary
This gene has 3 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000307296.8 PDCD6IP-201 5884 868aa Protein coding CCDS2660 Q8WUM4 NM_013374.6 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P2, TSL:1,
ENST00000457054.6 PDCD6IP-206 5962 873aa Protein coding CCDS54561 Q8WUM4-2 - GENCODE basic, APPRIS ALT1, TSL:1,
ENST00000413073.1 PDCD6IP-203 566 153aa Protein coding C9IZF9 - TSL:4, CDS 3' incomplete,
ENST00000648706.1 PDCD6IP-218 6049 300aa Nonsense mediated decay A0A3B3IT07 - -
ENST00000412887.5 PDCD6IP-202 842 95aa Nonsense mediated decay F8WDK9 - TSL:3,
ENST00000435909.5 PDCD6IP-205 587 72aa Nonsense mediated decay F8WEQ7 - TSL:5,
ENST00000430877.5 PDCD6IP-204 582 78aa Nonsense mediated decay F8WBR8 - TSL:3,
ENST00000498147.5 PDCD6IP-217 802 No protein Protein coding CDS not defined - - TSL:3,
ENST00000482561.1 PDCD6IP-211 626 No protein Protein coding CDS not defined - - TSL:5,
ENST00000484478.5 PDCD6IP-212 596 No protein Protein coding CDS not defined - - TSL:4,
ENST00000477798.5 PDCD6IP-210 587 No protein Protein coding CDS not defined - - TSL:4,
ENST00000495235.2 PDCD6IP-216 428 No protein Protein coding CDS not defined - - TSL:2,
ENST00000465122.5 PDCD6IP-208 894 No protein Retained intron - - TSL:3,
ENST00000489869.1 PDCD6IP-214 842 No protein Retained intron - - TSL:2,
ENST00000487821.1 PDCD6IP-213 750 No protein Retained intron - - TSL:4,
ENST00000459659.1 PDCD6IP-207 662 No protein Retained intron - - TSL:3,
ENST00000494810.1 PDCD6IP-215 619 No protein Retained intron - - TSL:3,
ENST00000473593.1 PDCD6IP-209 540 No protein Retained intron - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDCD6IP
This KO Strategy loading
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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