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CRISPR-U™ Gene Knockout Cell Line Strategy

HDAC5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human HDAC5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HDAC5
Gene id 10014
Organism Homo sapiens
Official full symbol histone deacetylase 5
Gene type protein-coding
Also known as HD5, NY-CO-9
Summary Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 17
Strategy Summary
This gene has 8 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000682912.1 HDAC5-213 5319 1122aa Protein coding CCDS45696 Q9UQL6 NM_005474.5 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P4,
ENST00000225983.10 HDAC5-201 5326 1123aa Protein coding CCDS32663 Q9UQL6-3 - GENCODE basic, APPRIS ALT2, TSL:5,
ENST00000336057.9 HDAC5-202 5040 1037aa Protein coding Q9UQL6-2 - GENCODE basic, TSL:1,
ENST00000586802.5 HDAC5-204 3662 1122aa Protein coding CCDS45696 Q9UQL6 - GENCODE basic, APPRIS P4, TSL:1,
ENST00000588261.1 HDAC5-207 846 282aa Protein coding K7EJL4 - TSL:3, CDS 5' and 3' incomplete,
ENST00000591714.5 HDAC5-210 667 68aa Protein coding K7ES01 - TSL:3, CDS 3' incomplete,
ENST00000587135.1 HDAC5-205 504 40aa Protein coding K7EJZ7 - TSL:2, CDS 3' incomplete,
ENST00000588703.5 HDAC5-209 445 79aa Protein coding K7EJL6 - TSL:3, CDS 3' incomplete,
ENST00000592385.5 HDAC5-211 2601 No protein Retained intron - - TSL:5,
ENST00000593013.5 HDAC5-212 1745 No protein Retained intron - - TSL:2,
ENST00000587776.1 HDAC5-206 917 No protein Retained intron - - TSL:3,
ENST00000588419.1 HDAC5-208 742 No protein Retained intron - - TSL:2,
ENST00000586339.1 HDAC5-203 465 No protein Retained intron - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HDAC5
This KO Strategy loading
Red Cotton™ Notes Gene HDAC5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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