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CRISPR-U™ Gene Knockout Cell Line Strategy

AKT3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human AKT3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AKT3
Gene id 10000
Organism Homo sapiens
Official full symbol AKT serine/threonine kinase 3
Gene type protein-coding
Also known as MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Summary The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Genomic regions Chromosome 1
Strategy Summary
This gene has 11 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000673466.1 AKT3-217 7281 479aa Protein coding CCDS31077 Q9Y243 NM_005465.7 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1,
ENST00000366539.6 AKT3-203 4967 456aa Protein coding A0A5K1VW74 - GENCODE basic, TSL:5,
ENST00000672578.1 AKT3-214 4925 418aa Protein coding A0A5F9ZHU3 - GENCODE basic,
ENST00000672238.1 AKT3-211 3417 100aa Protein coding A0A7P0TB29 - GENCODE basic,
ENST00000492957.2 AKT3-208 3225 247aa Protein coding A0A7P0TBJ6 - GENCODE basic, TSL:5,
ENST00000336199.9 AKT3-202 1688 465aa Protein coding CCDS31076 Q9Y243-2 - GENCODE basic, TSL:1,
ENST00000263826.12 AKT3-201 1583 479aa Protein coding CCDS31077 Q9Y243 - GENCODE basic, APPRIS P1, TSL:1,
ENST00000366540.5 AKT3-204 1575 465aa Protein coding CCDS31076 Q9Y243-2 - GENCODE basic, TSL:1,
ENST00000680056.1 AKT3-219 1314 437aa Protein coding A0A7P0T8T9 - GENCODE basic,
ENST00000491219.6 AKT3-207 602 71aa Protein coding A0A5F9ZGZ4 - TSL:3, CDS 5' incomplete,
ENST00000673400.1 AKT3-216 427 66aa Protein coding A0A7P0T872 - GENCODE basic,
ENST00000672442.1 AKT3-212 3415 94aa Nonsense mediated decay A0A5F9ZHA9 - -
ENST00000680118.1 AKT3-220 2374 479aa Nonsense mediated decay CCDS31077 Q9Y243 - -
ENST00000672460.1 AKT3-213 2072 398aa Nonsense mediated decay A0A5F9ZGY0 - CDS 5' incomplete,
ENST00000681794.1 AKT3-222 1543 295aa Nonsense mediated decay A0A7P0TB32 - -
ENST00000490018.1 AKT3-206 664 No protein Protein coding CDS not defined - - TSL:3,
ENST00000672679.1 AKT3-215 589 No protein Protein coding CDS not defined - - -
ENST00000550388.1 AKT3-209 572 No protein Protein coding CDS not defined - - TSL:4,
ENST00000463991.5 AKT3-205 422 No protein Protein coding CDS not defined - - TSL:3,
ENST00000679831.1 AKT3-218 7950 No protein Retained intron - - -
ENST00000681055.1 AKT3-221 5343 No protein Retained intron - - -
ENST00000552631.2 AKT3-210 557 No protein Retained intron - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AKT3
This KO Strategy loading
Red Cotton™ Notes Gene AKT3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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