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CRISPR-U™ Gene Knockout Cell Line Strategy

CDH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human CDH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDH2
Gene id 1000
Organism Homo sapiens
Official full symbol cadherin 2
Gene type protein-coding
Also known as ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD
Summary This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Genomic regions Chromosome 18
Strategy Summary
This gene has 7 protein coding transcripts:
Transcript ID Name bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ENST00000269141.8 CDH2-201 4016 906aa Protein coding CCDS11891 A0A024RC42 P19022 NM_001792.5 MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1, TSL:1,
ENST00000399380.7 CDH2-202 3737 875aa Protein coding CCDS77172 P19022-2 - GENCODE basic, TSL:2,
ENST00000676445.1 CDH2-211 3243 821aa Protein coding C9J126 - GENCODE basic,
ENST00000430882.6 CDH2-205 3143 821aa Protein coding C9J126 - TSL:4,
ENST00000413878.2 CDH2-203 2574 691aa Protein coding C9JMH2 - TSL:4, CDS 3' incomplete,
ENST00000675173.1 CDH2-207 1397 394aa Protein coding A0A6Q8PHJ8 - CDS 5' incomplete,
ENST00000418492.5 CDH2-204 740 191aa Protein coding C9J8J8 - TSL:5, CDS 3' incomplete,
ENST00000675708.1 CDH2-209 3860 733aa Nonsense mediated decay A0A6Q8PF58 - -
ENST00000675688.1 CDH2-208 899 161aa Nonsense mediated decay A0A6Q8PG20 - CDS 5' incomplete,
ENST00000674998.1 CDH2-206 3627 No protein Retained intron - - -
ENST00000676041.1 CDH2-210 2947 No protein Retained intron - - -
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDH2
This KO Strategy loading
Red Cotton™ Notes Gene CDH2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$80

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Ubigene, make genome editing easier

CRISPR-U™ Technology

CRISPR-U™ technology is the exclusive gene-editing cell technology patent of Ubigene. With CRISPR-U™, Ubigene has successfully modified over 5000 genes from more than 200 mammalian cell lines including iPS/H1/H9 in the continuous upgrading process, and the KO efficiency 10~20-fold higher than conventional technology. Not only with higher cutting efficiency, but higher homologous recombination efficiency, CRISPR-U™ can achieve complete KO, point mutation and KI in cell lines. At present, Ubigene has successfully established a Cell Line Bank containing 3000+ off-shelf KO cells.

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EZ-editor™ Gene-editing Products

Ubigene’s expert team has exclusively innovated and developed products, including cell lines, cell culture medium, kits, gRNA plasmids, off-shelf lentivirus, etc, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!Currently hot-sales: Over 10,000 in-stock gRNA plasmids, >in-stock Cas9/Luc/EGFP Stable Cell Lines, Monoclone Validation Kit (extraction free), Cell Monoclonal Culture Medium, Transfection Culture Medium......

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Ubigene focuses on gene editing and has rich experience in cell line gene editing. Ubigene provides 8 major off-shelf libraries e.g. human/mouse genome-wide, as well as kinase-, cell cycle-, membrane protein-, and metabolic gene-related CRISPR knockout gRNA libraries, and one-stop services such as sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis. Ubigene offers multiple deliverables to meet different research needs.

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