CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 200 types of cell lines.
Objective
To create a Human CILK1 Knockout
model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol
CILK1
Gene id
22858
Organism
Homo sapiens
Gene type
protein-coding
Official full symbol
ciliogenesis associated kinase 1
Also known as
ECO, EJM10, ICK, LCK2, MRK, hICK
Genomic regions
Chromosome 6
Summary
Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified.
Strategy Summary
This gene has 3 protein coding transcripts:
Transcript ID
Name
bp
Protein
Biotype
CCDS
UniProt Match
RefSeq Match
Flags
ENST00000676107.1
CILK1-203
6146
632aa
Protein coding
CCDS4949
Q9UPZ9-1
NM_014920.5
MANE Select, Ensembl Canonical, GENCODE basic, APPRIS P1,
ENST00000356971.3
CILK1-202
6227
632aa
Protein coding
CCDS4949
Q9UPZ9-1
-
GENCODE basic, APPRIS P1, TSL:2,
ENST00000350082.10
CILK1-201
6137
639aa
Protein coding
CCDS93933
A0A7I2PIU1
-
GENCODE basic, TSL:1,
Frame-shift
Fragment A
Fragment B
gRNA Detail
Strategy
Project Comprehensive Difficulty Assessment
According to the Red Cotton database: the CRISPR gene-editing strategy design is Unknown. Knockout project comprehensive difficulty is thus assessed as Unknown.
EZ-editor™ Gene Dependency
EZ-editor™ Gene Dependency
Result
The CILK1 gene you inquire is evaluated as high risk
in 0%
cell line.
Cell line is not selected, unable to assess the accurate risk level, for reference only.
Ubigene is an international high-technology enterprise focused on gene-editing cells. Our exclusive CRISPR-U™ technology has 10-20 times more efficient editing than traditional methods, easily achieving gene knockout, point mutation, and knock-in. Based on CRISPR-U™ technology, Ubigene has accumulated over 5000 successful gene-editing cases from more than 200 cell lines including iPSC and ESC, and has established a KO Cell Line Bank with 3500+ KO cell lines and Red Cotton™ gRNA Plasmid Bank with 10000+ gRNA plasmids available in stock.
Ubigene focuses on technological innovation and product development, of which EZ-editor™ series products that cover the whole workflow of gene-editing keep improving. Ubigene will move on toward our goal of "Make genome editing easier" and we won't stop!